PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Zoysia matrella
FAR1 Family
Species TF ID Description
Zmw_sc00058.1.g00170.1FAR1 family protein
Zmw_sc00063.1.g00370.1FAR1 family protein
Zmw_sc00224.1.g00040.1FAR1 family protein
Zmw_sc00232.1.g00530.1FAR1 family protein
Zmw_sc00355.1.g00480.1FAR1 family protein
Zmw_sc00583.1.g00380.1FAR1 family protein
Zmw_sc00654.1.g00280.1FAR1 family protein
Zmw_sc00680.1.g00170.1FAR1 family protein
Zmw_sc01003.1.g00120.1FAR1 family protein
Zmw_sc01017.1.g00070.1FAR1 family protein
Zmw_sc01091.1.g00230.1FAR1 family protein
Zmw_sc01305.1.g00130.1FAR1 family protein
Zmw_sc01379.1.g00150.1FAR1 family protein
Zmw_sc01461.1.g00070.1FAR1 family protein
Zmw_sc01613.1.g00280.1FAR1 family protein
Zmw_sc02069.1.g00140.1FAR1 family protein
Zmw_sc02218.1.g00070.1FAR1 family protein
Zmw_sc02403.1.g00070.1FAR1 family protein
Zmw_sc02405.1.g00040.1FAR1 family protein
Zmw_sc02654.1.g00130.1FAR1 family protein
Zmw_sc02689.1.g00060.1FAR1 family protein
Zmw_sc02959.1.g00050.1FAR1 family protein
Zmw_sc02985.1.g00050.1FAR1 family protein
Zmw_sc03262.1.g00080.1FAR1 family protein
Zmw_sc03482.1.g00100.1FAR1 family protein
Zmw_sc04738.1.g00010.1FAR1 family protein
Zmw_sc04921.1.g00010.1FAR1 family protein
Zmw_sc05780.1.g00010.1FAR1 family protein
Zmw_sc05914.1.g00010.1FAR1 family protein
Zmw_sc07242.1.g00020.1FAR1 family protein
Zmw_sc08148.1.g00010.1FAR1 family protein
Zmw_sc09157.1.g00010.1FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885