PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Theobroma cacao
HSF Family
Species TF ID Description
Thecc1EG000610t1HSF family protein
Thecc1EG001784t1HSF family protein
Thecc1EG004275t1HSF family protein
Thecc1EG004275t2HSF family protein
Thecc1EG004275t3HSF family protein
Thecc1EG004275t4HSF family protein
Thecc1EG004275t5HSF family protein
Thecc1EG004275t6HSF family protein
Thecc1EG004275t7HSF family protein
Thecc1EG004275t8HSF family protein
Thecc1EG011279t1HSF family protein
Thecc1EG015119t1HSF family protein
Thecc1EG016198t1HSF family protein
Thecc1EG016563t1HSF family protein
Thecc1EG016563t2HSF family protein
Thecc1EG020222t1HSF family protein
Thecc1EG020700t1HSF family protein
Thecc1EG022446t1HSF family protein
Thecc1EG024565t1HSF family protein
Thecc1EG025482t1HSF family protein
Thecc1EG029630t1HSF family protein
Thecc1EG029886t1HSF family protein
Thecc1EG033816t1HSF family protein
Thecc1EG037308t1HSF family protein
Thecc1EG037308t2HSF family protein
Thecc1EG037308t3HSF family protein
Thecc1EG037308t4HSF family protein
Thecc1EG037635t1HSF family protein
Thecc1EG037888t1HSF family protein
Thecc1EG040889t1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645