PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Prunus mume
HSF Family
Species TF ID Description
XP_008220242.1HSF family protein
XP_008220492.1HSF family protein
XP_008220924.1HSF family protein
XP_008222805.1HSF family protein
XP_008224242.1HSF family protein
XP_008225084.1HSF family protein
XP_008225237.1HSF family protein
XP_008226279.1HSF family protein
XP_008228879.1HSF family protein
XP_008234272.1HSF family protein
XP_008235357.1HSF family protein
XP_008238205.1HSF family protein
XP_008238807.1HSF family protein
XP_008238808.1HSF family protein
XP_008241431.1HSF family protein
XP_008241456.1HSF family protein
XP_008242372.1HSF family protein
XP_008242623.1HSF family protein
XP_008244246.1HSF family protein
XP_016650969.1HSF family protein
XP_016650970.1HSF family protein
XP_016650971.1HSF family protein
XP_016650972.1HSF family protein
XP_016650973.1HSF family protein
XP_016650974.1HSF family protein
XP_016651915.1HSF family protein
XP_016651916.1HSF family protein
XP_016651917.1HSF family protein
XP_016651918.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645