PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Populus euphratica
ZF-HD Family
Species TF ID Description
CCG000343.1ZF-HD family protein
CCG002067.1ZF-HD family protein
CCG002275.1ZF-HD family protein
CCG002814.1ZF-HD family protein
CCG003039.1ZF-HD family protein
CCG005285.1ZF-HD family protein
CCG008927.1ZF-HD family protein
CCG014782.1ZF-HD family protein
CCG018584.1ZF-HD family protein
CCG018623.1ZF-HD family protein
CCG021469.1ZF-HD family protein
CCG021658.1ZF-HD family protein
CCG022238.1ZF-HD family protein
CCG025428.1ZF-HD family protein
CCG026445.1ZF-HD family protein
CCG026650.1ZF-HD family protein
CCG027561.1ZF-HD family protein
CCG028612.1ZF-HD family protein
CCG028987.1ZF-HD family protein
CCG029837.1ZF-HD family protein
CCG030309.1ZF-HD family protein
CCG031259.1ZF-HD family protein
ZF-HD Family Introduction

This group of sequences described by a 54-residue domain found in the N-terminal region of plant proteins, the vast majority of which contain a ZF-HD class homeobox domain toward the C terminus. The region between the two domains typically is rich in low complexity sequence. The companion ZF-HD homeobox domain is described in INTERPRO:IPR006455.

A one-hybrid screen resulted in the cloning of four different members of a novel class of plant homeodomain proteins, which are most likely involved in the mesophyll-specific expression of the C4 PEPCase gene in C4 species of the genus Flaveria. Inspection of the homeodomains of the four proteins reveals that they share many common features with homeodomains described so far, but there are also significant differences. Interestingly, this class of homeodomain proteins occurs also in Arabidopsis thaliana and other C3 plants. One-hybrid experiments as well as in vitro DNA binding studies confirmed that these novel homeodomain proteins specifically interact with the proximal region of the C4 PEPCase (C4 phosphoenolpyruvate carboxylase) gene. The N-terminal domains of the homeodomain proteins contain highly conserved sequence motifs. Two-hybrid experiments show that these motifs are sufficient to confer homo- or heterodimer formation between the proteins. Mutagenesis of conserved cysteine residues within the dimerization domain indicates that these residues are essential for dimer formation. Therefore, we designate this novel class of homeobox proteins ZF-HD, for zinc finger homeodomain protein. Our data suggest that the ZF-HD class of homeodomain proteins may be involved in the establishment of the characteristic expression pattern of the C4 PEPCase gene.

Windhovel A, Hein I, Dabrowa R, Stockhaus J.
Characterization of a novel class of plant homeodomain proteins that bind to the C4 phosphoenolpyruvate carboxylase gene of Flaveria trinervia.
Plant Mol Biol. 2001 Jan;45(2):201-14.
PMID: 11289511