PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Populus euphratica
HSF Family
Species TF ID Description
CCG000940.1HSF family protein
CCG001341.1HSF family protein
CCG001458.1HSF family protein
CCG001724.1HSF family protein
CCG004861.1HSF family protein
CCG005948.1HSF family protein
CCG006244.1HSF family protein
CCG007214.1HSF family protein
CCG007863.1HSF family protein
CCG009216.1HSF family protein
CCG009416.1HSF family protein
CCG010253.1HSF family protein
CCG013195.1HSF family protein
CCG013896.1HSF family protein
CCG014424.1HSF family protein
CCG015090.1HSF family protein
CCG017804.1HSF family protein
CCG019200.1HSF family protein
CCG019201.1HSF family protein
CCG020996.1HSF family protein
CCG023235.1HSF family protein
CCG023633.1HSF family protein
CCG024466.1HSF family protein
CCG026694.1HSF family protein
CCG027759.1HSF family protein
CCG030319.1HSF family protein
CCG032077.1HSF family protein
CCG033049.1HSF family protein
CCG033925.1HSF family protein
CCG034159.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645