PlantRegMap/PlantTFDB v5.0
Plant Transcription
Factor Database
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Home TFext BLAST Prediction Download Help About Links PlantRegMap |
Species | TF ID | Description |
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AMDW01034480.1_FGP001 | FAR1 family protein | |
AMDW01035649.1_FGP001 | FAR1 family protein | |
AMDW01037255.1_FGP001 | FAR1 family protein | |
AMDW01040774.1_FGP001 | FAR1 family protein | |
KN538681.1_FGP300 | FAR1 family protein | |
KN538688.1_FGP103 | FAR1 family protein | |
KN538688.1_FGP146 | FAR1 family protein | |
KN538709.1_FGP108 | FAR1 family protein | |
KN538779.1_FGP003 | FAR1 family protein | |
KN538859.1_FGP013 | FAR1 family protein | |
KN538871.1_FGP005 | FAR1 family protein | |
KN538893.1_FGP004 | FAR1 family protein | |
KN538917.1_FGP001 | FAR1 family protein | |
KN538918.1_FGP012 | FAR1 family protein | |
KN538930.1_FGP009 | FAR1 family protein | |
KN539106.1_FGP020 | FAR1 family protein | |
KN539262.1_FGP008 | FAR1 family protein | |
KN539403.1_FGP013 | FAR1 family protein | |
KN539430.1_FGP005 | FAR1 family protein | |
KN539566.1_FGP003 | FAR1 family protein | |
KN539590.1_FGP003 | FAR1 family protein | |
KN539644.1_FGP008 | FAR1 family protein | |
KN539852.1_FGP007 | FAR1 family protein | |
KN539882.1_FGP003 | FAR1 family protein | |
KN540439.1_FGP001 | FAR1 family protein | |
KN540502.1_FGP001 | FAR1 family protein | |
KN540508.1_FGP001 | FAR1 family protein | |
KN540735.1_FGP001 | FAR1 family protein | |
KN540758.1_FGP001 | FAR1 family protein | |
KN541071.1_FGP002 | FAR1 family protein | |
KN541160.1_FGP002 | FAR1 family protein | |
KN542241.1_FGP002 | FAR1 family protein | |
KN542275.1_FGP001 | FAR1 family protein | |
KN542312.1_FGP001 | FAR1 family protein | |
KN542337.1_FGP002 | FAR1 family protein | |
KN542505.1_FGP002 | FAR1 family protein | |
KN542584.1_FGP001 | FAR1 family protein | |
KN542610.1_FGP002 | FAR1 family protein |
We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885