PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Oryza brachyantha
HSF Family
Species TF ID Description
OB01G29910.1HSF family protein
OB01G32770.1HSF family protein
OB01G38820.1HSF family protein
OB01G39790.1HSF family protein
OB02G18670.1HSF family protein
OB02G25250.1HSF family protein
OB03G14700.1HSF family protein
OB03G19170.1HSF family protein
OB03G27910.1HSF family protein
OB03G40890.1HSF family protein
OB03G44190.1HSF family protein
OB04G29050.1HSF family protein
OB05G31230.1HSF family protein
OB06G25820.1HSF family protein
OB06G26400.1HSF family protein
OB08G24900.1HSF family protein
OB08G29340.1HSF family protein
OB09G19080.1HSF family protein
OB09G19180.1HSF family protein
OB10G18020.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645