PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Morus notabilis
HSF Family
Species TF ID Description
XP_010087101.1HSF family protein
XP_010088011.1HSF family protein
XP_010088821.1HSF family protein
XP_010088963.1HSF family protein
XP_010090068.1HSF family protein
XP_010092004.1HSF family protein
XP_010093311.1HSF family protein
XP_010093647.1HSF family protein
XP_010096858.1HSF family protein
XP_010096952.1HSF family protein
XP_010097048.1HSF family protein
XP_010097956.1HSF family protein
XP_010105217.1HSF family protein
XP_010106176.1HSF family protein
XP_010107420.1HSF family protein
XP_010107707.1HSF family protein
XP_010108202.1HSF family protein
XP_010109197.1HSF family protein
XP_010112180.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645