PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Lotus japonicus
FAR1 Family
Species TF ID Description
Lj0g3v0059989.1FAR1 family protein
Lj0g3v0085139.1FAR1 family protein
Lj0g3v0136299.1FAR1 family protein
Lj0g3v0154199.3FAR1 family protein
Lj0g3v0215159.1FAR1 family protein
Lj0g3v0215159.2FAR1 family protein
Lj0g3v0215159.3FAR1 family protein
Lj0g3v0224509.1FAR1 family protein
Lj0g3v0232569.1FAR1 family protein
Lj0g3v0249079.1FAR1 family protein
Lj0g3v0304669.1FAR1 family protein
Lj0g3v0321259.1FAR1 family protein
Lj0g3v0363549.1FAR1 family protein
Lj1g3v0162460.1FAR1 family protein
Lj1g3v0162460.2FAR1 family protein
Lj1g3v1932810.1FAR1 family protein
Lj1g3v3556900.1FAR1 family protein
Lj1g3v4251280.1FAR1 family protein
Lj1g3v4467990.1FAR1 family protein
Lj2g3v1573090.1FAR1 family protein
Lj2g3v1573090.2FAR1 family protein
Lj2g3v1695250.2FAR1 family protein
Lj2g3v3413360.1FAR1 family protein
Lj3g3v1953520.1FAR1 family protein
Lj3g3v1953520.2FAR1 family protein
Lj4g3v0070730.1FAR1 family protein
Lj4g3v0070880.1FAR1 family protein
Lj4g3v0119630.1FAR1 family protein
Lj4g3v0120440.1FAR1 family protein
Lj4g3v0620710.1FAR1 family protein
Lj4g3v0654370.1FAR1 family protein
Lj4g3v2120630.1FAR1 family protein
Lj5g3v0309090.1FAR1 family protein
Lj5g3v2110900.1FAR1 family protein
Lj6g3v1038750.1FAR1 family protein
Lj6g3v1038750.2FAR1 family protein
Lj6g3v1692720.1FAR1 family protein
Lj6g3v1692830.1FAR1 family protein
Lj6g3v1722710.1FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885