PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Jatropha curcas
FAR1 Family
Species TF ID Description
Jcr4S00002.110FAR1 family protein
Jcr4S00007.210FAR1 family protein
Jcr4S00028.120FAR1 family protein
Jcr4S00050.50FAR1 family protein
Jcr4S00050.60FAR1 family protein
Jcr4S00050.70FAR1 family protein
Jcr4S00092.110FAR1 family protein
Jcr4S00206.70FAR1 family protein
Jcr4S00363.10FAR1 family protein
Jcr4S00363.40FAR1 family protein
Jcr4S00363.50FAR1 family protein
Jcr4S00363.90FAR1 family protein
Jcr4S00586.70FAR1 family protein
Jcr4S00606.70FAR1 family protein
Jcr4S00635.50FAR1 family protein
Jcr4S00715.100FAR1 family protein
Jcr4S00856.50FAR1 family protein
Jcr4S01124.70FAR1 family protein
Jcr4S01444.60FAR1 family protein
Jcr4S01879.40FAR1 family protein
Jcr4S02431.30FAR1 family protein
Jcr4S02686.40FAR1 family protein
Jcr4S02818.20FAR1 family protein
Jcr4S03358.30FAR1 family protein
Jcr4S03358.50FAR1 family protein
Jcr4S03657.10FAR1 family protein
Jcr4S04285.70FAR1 family protein
Jcr4S06752.20FAR1 family protein
Jcr4S07969.10FAR1 family protein
Jcr4S08056.40FAR1 family protein
Jcr4S09176.10FAR1 family protein
Jcr4S09723.10FAR1 family protein
Jcr4S10148.10FAR1 family protein
Jcr4S11691.20FAR1 family protein
Jcr4S11691.40FAR1 family protein
Jcr4S12774.10FAR1 family protein
Jcr4S13052.10FAR1 family protein
Jcr4S18792.20FAR1 family protein
Jcr4S25889.10FAR1 family protein
Jcr4S28438.10FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885