PlantRegMap/PlantTFDB v5.0
Plant Transcription
Factor Database
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Home TFext BLAST Prediction Download Help About Links PlantRegMap |
Species | TF ID | Description |
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Glycine soja (60) | KHN02803.1 | FAR1 family protein |
KHN06125.1 | FAR1 family protein | |
KHN06202.1 | FAR1 family protein | |
KHN06792.1 | FAR1 family protein | |
KHN07370.1 | FAR1 family protein | |
KHN10095.1 | FAR1 family protein | |
KHN10135.1 | FAR1 family protein | |
KHN10555.1 | FAR1 family protein | |
KHN10918.1 | FAR1 family protein | |
KHN11326.1 | FAR1 family protein | |
KHN12906.1 | FAR1 family protein | |
KHN13933.1 | FAR1 family protein | |
KHN14374.1 | FAR1 family protein | |
KHN14375.1 | FAR1 family protein | |
KHN14976.1 | FAR1 family protein | |
KHN15674.1 | FAR1 family protein | |
KHN18250.1 | FAR1 family protein | |
KHN19983.1 | FAR1 family protein | |
KHN20508.1 | FAR1 family protein | |
KHN20602.1 | FAR1 family protein | |
KHN20822.1 | FAR1 family protein | |
KHN22170.1 | FAR1 family protein | |
KHN22392.1 | FAR1 family protein | |
KHN22805.1 | FAR1 family protein | |
KHN24804.1 | FAR1 family protein | |
KHN25102.1 | FAR1 family protein | |
KHN25297.1 | FAR1 family protein | |
KHN25302.1 | FAR1 family protein | |
KHN26435.1 | FAR1 family protein | |
KHN26808.1 | FAR1 family protein | |
KHN30100.1 | FAR1 family protein | |
KHN30121.1 | FAR1 family protein | |
KHN31358.1 | FAR1 family protein | |
KHN31427.1 | FAR1 family protein | |
KHN31867.1 | FAR1 family protein | |
KHN32681.1 | FAR1 family protein | |
KHN32934.1 | FAR1 family protein | |
KHN33085.1 | FAR1 family protein | |
KHN33306.1 | FAR1 family protein | |
KHN33310.1 | FAR1 family protein | |
KHN34514.1 | FAR1 family protein | |
KHN34778.1 | FAR1 family protein | |
KHN35527.1 | FAR1 family protein | |
KHN37548.1 | FAR1 family protein | |
KHN38124.1 | FAR1 family protein | |
KHN38445.1 | FAR1 family protein | |
KHN39540.1 | FAR1 family protein | |
KHN39704.1 | FAR1 family protein | |
KHN40737.1 | FAR1 family protein | |
KHN40804.1 | FAR1 family protein | |
KHN40913.1 | FAR1 family protein | |
KHN41795.1 | FAR1 family protein | |
KHN42330.1 | FAR1 family protein | |
KHN42873.1 | FAR1 family protein | |
KHN42936.1 | FAR1 family protein | |
KHN42959.1 | FAR1 family protein | |
KHN43827.1 | FAR1 family protein | |
KHN46338.1 | FAR1 family protein | |
KHN46858.1 | FAR1 family protein | |
KHN48773.1 | FAR1 family protein |
We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885