PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Fragaria vesca
HSF Family
Species TF ID Description
mrna02408.1-v1.0-hybridHSF family protein
mrna02464.1-v1.0-hybridHSF family protein
mrna02705.1-v1.0-hybridHSF family protein
mrna06570.1-v1.0-hybridHSF family protein
mrna10474.1-v1.0-hybridHSF family protein
mrna12667.1-v1.0-hybridHSF family protein
mrna13301.1-v1.0-hybridHSF family protein
mrna13904.1-v1.0-hybridHSF family protein
mrna20347.1-v1.0-hybridHSF family protein
mrna23802.1-v1.0-hybridHSF family protein
mrna24036.1-v1.0-hybridHSF family protein
mrna29004.1-v1.0-hybridHSF family protein
mrna30146.1-v1.0-hybridHSF family protein
mrna30881.1-v1.0-hybridHSF family protein
mrna32416.1-v1.0-hybridHSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645