PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Eutrema salsugineum
HSF Family
Species TF ID Description
Thhalv10000950mHSF family protein
Thhalv10002333mHSF family protein
Thhalv10002872mHSF family protein
Thhalv10003365mHSF family protein
Thhalv10003411mHSF family protein
Thhalv10004690mHSF family protein
Thhalv10005251mHSF family protein
Thhalv10006463mHSF family protein
Thhalv10011051mHSF family protein
Thhalv10012033mHSF family protein
Thhalv10012073mHSF family protein
Thhalv10013428mHSF family protein
Thhalv10013758mHSF family protein
Thhalv10015013mHSF family protein
Thhalv10015077mHSF family protein
Thhalv10017795mHSF family protein
Thhalv10018723mHSF family protein
Thhalv10020648mHSF family protein
Thhalv10020829mHSF family protein
Thhalv10025090mHSF family protein
Thhalv10025116mHSF family protein
Thhalv10025409mHSF family protein
Thhalv10025906mHSF family protein
Thhalv10026540mHSF family protein
Thhalv10026981mHSF family protein
Thhalv10027510mHSF family protein
Thhalv10028026mHSF family protein
Thhalv10028027mHSF family protein
Thhalv10028744mHSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645