PlantRegMap/PlantTFDB v5.0
Plant Transcription
Factor Database
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Home TFext BLAST Prediction Download Help About Links PlantRegMap |
Species | TF ID | Description |
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Eucgr.A00087.1.p | FAR1 family protein | |
Eucgr.A00281.1.p | FAR1 family protein | |
Eucgr.A00281.2.p | FAR1 family protein | |
Eucgr.A00281.3.p | FAR1 family protein | |
Eucgr.A00908.1.p | FAR1 family protein | |
Eucgr.A00908.2.p | FAR1 family protein | |
Eucgr.A00908.3.p | FAR1 family protein | |
Eucgr.A00910.1.p | FAR1 family protein | |
Eucgr.A00910.2.p | FAR1 family protein | |
Eucgr.B00042.1.p | FAR1 family protein | |
Eucgr.B01259.1.p | FAR1 family protein | |
Eucgr.B01589.1.p | FAR1 family protein | |
Eucgr.B01750.1.p | FAR1 family protein | |
Eucgr.B01751.1.p | FAR1 family protein | |
Eucgr.B03670.1.p | FAR1 family protein | |
Eucgr.C01967.1.p | FAR1 family protein | |
Eucgr.C02098.1.p | FAR1 family protein | |
Eucgr.C03166.1.p | FAR1 family protein | |
Eucgr.C04090.1.p | FAR1 family protein | |
Eucgr.C04090.2.p | FAR1 family protein | |
Eucgr.D00033.1.p | FAR1 family protein | |
Eucgr.D01451.1.p | FAR1 family protein | |
Eucgr.D01455.1.p | FAR1 family protein | |
Eucgr.E01219.1.p | FAR1 family protein | |
Eucgr.E01986.1.p | FAR1 family protein | |
Eucgr.F00557.1.p | FAR1 family protein | |
Eucgr.F03610.1.p | FAR1 family protein | |
Eucgr.F03904.1.p | FAR1 family protein | |
Eucgr.G00085.1.p | FAR1 family protein | |
Eucgr.G00741.1.p | FAR1 family protein | |
Eucgr.H00706.1.p | FAR1 family protein | |
Eucgr.H00708.1.p | FAR1 family protein | |
Eucgr.H00883.1.p | FAR1 family protein | |
Eucgr.H01407.1.p | FAR1 family protein | |
Eucgr.I00824.1.p | FAR1 family protein | |
Eucgr.I00868.1.p | FAR1 family protein | |
Eucgr.I01547.1.p | FAR1 family protein | |
Eucgr.I01547.2.p | FAR1 family protein | |
Eucgr.J01116.1.p | FAR1 family protein | |
Eucgr.J03074.1.p | FAR1 family protein | |
Eucgr.J03076.1.p | FAR1 family protein | |
Eucgr.J03078.1.p | FAR1 family protein | |
Eucgr.K02085.1.p | FAR1 family protein | |
Eucgr.K03522.1.p | FAR1 family protein | |
Eucgr.L00168.1.p | FAR1 family protein | |
Eucgr.L03237.1.p | FAR1 family protein |
We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885