PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Eucalyptus camaldulensis
HSF Family
Species TF ID Description
EcC000566.10HSF family protein
EcC005220.10HSF family protein
EcC012707.10HSF family protein
EcC014478.20HSF family protein
EcC016109.10HSF family protein
EcC022141.10HSF family protein
EcC031900.20HSF family protein
EcC031945.20HSF family protein
EcC033061.10HSF family protein
EcC033686.50HSF family protein
EcC036324.10HSF family protein
EcC041299.10HSF family protein
EcC042361.20HSF family protein
EcC045068.20HSF family protein
EcC046342.20HSF family protein
EcC047704.10HSF family protein
EcC048466.30HSF family protein
EcC052629.20HSF family protein
EcC054130.30HSF family protein
EcC054761.90HSF family protein
EcC055204.10HSF family protein
EcC055204.130HSF family protein
EcC055204.150HSF family protein
EcC055204.60HSF family protein
EcC060749.10HSF family protein
EcC079046.10HSF family protein
EcC079630.10HSF family protein
EcS670990.10HSF family protein
EcS752808.10HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645