PlantRegMap/PlantTFDB v5.0
Plant Transcription
Factor Database
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Home TFext BLAST Prediction Download Help About Links PlantRegMap |
Species | TF ID | Description |
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EcC001316.20 | FAR1 family protein | |
EcC001753.10 | FAR1 family protein | |
EcC003157.10 | FAR1 family protein | |
EcC006223.20 | FAR1 family protein | |
EcC006223.40 | FAR1 family protein | |
EcC006223.60 | FAR1 family protein | |
EcC006588.20 | FAR1 family protein | |
EcC007322.40 | FAR1 family protein | |
EcC007367.30 | FAR1 family protein | |
EcC009842.30 | FAR1 family protein | |
EcC013663.30 | FAR1 family protein | |
EcC015072.40 | FAR1 family protein | |
EcC015787.10 | FAR1 family protein | |
EcC017141.20 | FAR1 family protein | |
EcC020645.20 | FAR1 family protein | |
EcC027143.20 | FAR1 family protein | |
EcC032205.20 | FAR1 family protein | |
EcC032446.10 | FAR1 family protein | |
EcC033088.20 | FAR1 family protein | |
EcC034086.30 | FAR1 family protein | |
EcC045276.30 | FAR1 family protein | |
EcC046871.50 | FAR1 family protein | |
EcC047086.40 | FAR1 family protein | |
EcC052648.30 | FAR1 family protein | |
EcC053935.30 | FAR1 family protein | |
EcC053990.70 | FAR1 family protein | |
EcC053990.90 | FAR1 family protein | |
EcC054762.100 | FAR1 family protein | |
EcC055193.10 | FAR1 family protein | |
EcC066213.10 | FAR1 family protein | |
EcC073440.10 | FAR1 family protein | |
EcC076057.10 | FAR1 family protein | |
EcC081225.10 | FAR1 family protein | |
EcS723945.10 | FAR1 family protein | |
EcS727201.10 | FAR1 family protein | |
EcS729352.10 | FAR1 family protein | |
EcS733210.10 | FAR1 family protein | |
EcS737708.10 | FAR1 family protein | |
EcS771492.10 | FAR1 family protein |
We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885