PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Citrus clementina
FAR1 Family
Species TF ID Description
Ciclev10000252mFAR1 family protein
Ciclev10000255mFAR1 family protein
Ciclev10000383mFAR1 family protein
Ciclev10000456mFAR1 family protein
Ciclev10000496mFAR1 family protein
Ciclev10003358mFAR1 family protein
Ciclev10004376mFAR1 family protein
Ciclev10004395mFAR1 family protein
Ciclev10010226mFAR1 family protein
Ciclev10010993mFAR1 family protein
Ciclev10012372mFAR1 family protein
Ciclev10013881mFAR1 family protein
Ciclev10013892mFAR1 family protein
Ciclev10014295mFAR1 family protein
Ciclev10014297mFAR1 family protein
Ciclev10014474mFAR1 family protein
Ciclev10014479mFAR1 family protein
Ciclev10014481mFAR1 family protein
Ciclev10016335mFAR1 family protein
Ciclev10016336mFAR1 family protein
Ciclev10016654mFAR1 family protein
Ciclev10016721mFAR1 family protein
Ciclev10016736mFAR1 family protein
Ciclev10016948mFAR1 family protein
Ciclev10018084mFAR1 family protein
Ciclev10018239mFAR1 family protein
Ciclev10018266mFAR1 family protein
Ciclev10018310mFAR1 family protein
Ciclev10018794mFAR1 family protein
Ciclev10021841mFAR1 family protein
Ciclev10021846mFAR1 family protein
Ciclev10021853mFAR1 family protein
Ciclev10021886mFAR1 family protein
Ciclev10022205mFAR1 family protein
Ciclev10022249mFAR1 family protein
Ciclev10023561mFAR1 family protein
Ciclev10023742mFAR1 family protein
Ciclev10024022mFAR1 family protein
Ciclev10024302mFAR1 family protein
Ciclev10024536mFAR1 family protein
Ciclev10025157mFAR1 family protein
Ciclev10027335mFAR1 family protein
Ciclev10027619mFAR1 family protein
Ciclev10027888mFAR1 family protein
Ciclev10029941mFAR1 family protein
Ciclev10030151mFAR1 family protein
Ciclev10030197mFAR1 family protein
Ciclev10033450mFAR1 family protein
Ciclev10033711mFAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885