PlantRegMap/PlantTFDB v5.0
Plant Transcription Factor Database
Previous version: v3.0 v4.0
Ananas comosus
HSF Family
Species TF ID Description
Aco001320.1HSF family protein
Aco001332.1HSF family protein
Aco002684.1HSF family protein
Aco002688.1HSF family protein
Aco005573.1HSF family protein
Aco005592.1HSF family protein
Aco005862.1HSF family protein
Aco006046.1HSF family protein
Aco008819.1HSF family protein
Aco008972.1HSF family protein
Aco009685.1HSF family protein
Aco009991.1HSF family protein
Aco013873.1HSF family protein
Aco015210.1HSF family protein
Aco016114.1HSF family protein
Aco016689.1HSF family protein
Aco016980.1HSF family protein
Aco017163.1HSF family protein
Aco020850.1HSF family protein
Aco021879.1HSF family protein
Aco022474.1HSF family protein
Aco027352.1HSF family protein
Aco027680.1HSF family protein
Aco027746.1HSF family protein
Aco029409.1HSF family protein
Aco030273.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645